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Amoxicillin middle ear fluid penetration and pharmacokinetics in children with acute otitis media. The efficacy of antibiotic dosing regimens is usually assessed by antibiotic plasma pharmacokinetics or middle ear fluid (MEF) concentration at one or two time points. pharmacokinetic studies on antibiotic penetration into middle ear effusion (MEE) are difficult Key words: Otitis media, pharmacokinetics, middle ear, antibiotics.